India ink staining whole blood9/2/2023 ![]() ![]() Reconstruction and statistical analysis of the microvascular network, including derivation of quantitative vascular densities, indicate significant differences mainly in vessels with diameters less than 8 μm and large than 20 μm across different brain regions. The distributing patterns of the vascular system within the brain regions were acquired and our results show that the patterns of individual vessels are different from each other. Based on the brain-wide vascular spatial structures and brain regions indicated by cytoarchitecture in one and the same mouse brain, we reconstructed and annotated the vascular system atlas of both arteries and veins of the whole mouse brain for the first time. Using micro-optical sectioning tomography (MOST) with a modified Nissl staining method, we acquired five mouse brain data sets containing arteries, veins, and microvessels. Making a precise atlas for cerebral arteries and veins has been a century-old objective in neuroscience and neuropathology. Understanding amazingly complex brain functions and pathologies requires a complete cerebral vascular atlas in stereotaxic coordinates. 2MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.His one great achievement is being the father of three amazing children.Benyi Xiong 1,2 Anan Li 1,2 Yang Lou 1,2 Shangbin Chen 1,2 Ben Long 1,2 Jie Peng 1,2 Zhongqin Yang 1,2 Tonghui Xu 1,2 Xiaoquan Yang 1,2 Xiangning Li 1,2 Tao Jiang 1,2 Qingming Luo 1,2 Hui Gong 1,2 * He is one of the founders of the FOAM movement (Free Open-Access Medical education) and is co-creator of , the RAGE podcast, the Resuscitology course, and the SMACC conference. He created the ‘Critically Ill Airway’ course and teaches on numerous courses around the world. He coordinates the Alfred ICU’s education and simulation programmes and runs the unit’s education website, INTENSIVE. He is actively involved in in using translational simulation to improve patient care and the design of processes and systems at Alfred Health. He has completed fellowship training in both intensive care medicine and emergency medicine, as well as post-graduate training in biochemistry, clinical toxicology, clinical epidemiology, and health professional education. He is an internationally recognised Clinician Educator with a passion for helping clinicians learn and for improving the clinical performance of individuals and collectives.Īfter finishing his medical degree at the University of Auckland, he continued post-graduate training in New Zealand as well as Australia’s Northern Territory, Perth and Melbourne. He is on the Board of Directors for the Intensive Care Foundation and is a First Part Examiner for the College of Intensive Care Medicine. He is also a Clinical Adjunct Associate Professor at Monash University. He is a co-founder of the Australia and New Zealand Clinician Educator Network (ANZCEN) and is the Lead for the ANZCEN Clinician Educator Incubator programme. Anaerobes: Consider brain abscess, elderlyĬhris is an Intensivist and ECMO specialist at the Alfred ICU in Melbourne.H Influenzae: (3%) – Head trauma with CSF leak, otitis, sinusitis, anatomical defects such as dermal sinus tracts.Staphylococcus: Penetrating skull injury, ear or neuro operations.N meningitidis: (30%) – Children and adolescents.Pneumococcus: (40%) – Otitis media, head injury, pneumonia, immunocompromised.Lymphocytosis, variable protein elevation and normal glucose.Aseptic meningitis (Generally accepted as mainly viral meningitis).Sickle cell disease – Capsulated organisms.Humoral or asplenic – Neiserria, enterovirus.N meningitidis, s. pneumonia, listeria, klebsiella, s. aureus.extended culture (Listeria, Cryptococcus).xanthochromic index with spectrophotometry (in SAH).less than half serum in infections (bacterial, Tb and fungal infections) and vasculitis and sarcoidosis.increased in CNS inflammation (including CSF drains and blood in CSF).oligoclonal bands in multiple sclerosis.increased in GBS, vasculitis and sarcoidosis.increased in infection: Tb > bacterial > viral.mixed lymphocytosis/monocytosis in GBS and status epilepticus.lymphocytosis: viral, TB, cryptococcal and listerial infections.polymorphonuclear leukocytosis: bacterial infection.in traumatic tap classically taught to expect 1 WCC : 500 RCC (if normal in peripheral cell counts) but this is not reliable.yellow with xanthochromia (this takes 6-12 hours to develop after blood enters CSF).blood stained with SAH and traumatic taps.cryptoccal antigen and Indian ink stain. ![]()
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